103 research outputs found
Traffic by multiple species of molecular motors
We study the traffic of two types of molecular motors using the two-species
symmetric simple exclusion process (ASEP) with periodic boundary conditions and
with attachment and detachment of particles. We determine characteristic
properties such as motor densities and currents by simulations and analytical
calculations. For motors with different unbinding probabilities, mean field
theory gives the correct bound density and total current of the motors, as
shown by numerical simulations. For motors differing in their stepping
probabilities, the particle-hole symmetry of the current-density relationship
is broken and mean field theory fails drastically. The total motor current
exhibits exponential finite-size scaling, which we use to extrapolate the total
current to the thermodynamic limit. Finally, we also study the motion of a
single motor in the background of many non-moving motors.Comment: 23 pages, 6 figures, late
Molecular motor traffic in a half-open tube
The traffic of molecular motors which interact through mutual exclusion is
studied theoretically for half-open tube-like compartments. These half-open
tubes mimic the shapes of axons. The mutual exclusion leads to traffic jams or
density plateaus on the filaments. A phase transition is obtained when the
motor velocity changes sign. We identify the relevant length scales and
characterize the jamming behavior using both analytical approximations and
Monte Carlo simulations of lattice models.Comment: 14 pages, 5 postscript figure
Effects of dendritic core-shell glycoarchitectures on primary mesenchymal stem cells and osteoblasts obtained from different human donors
The biological impact of novel nano-scaled drug delivery vehicles in highly topical therapies of bone diseases have to be investigated in vitro before starting in vivo trials. Highly desired features for these materials are a good cellular uptake, large transport capacity for drugs and a good bio-compatibility. Essentially the latter has to be addressed as first point on the agenda. We present a study on the biological interaction of maltose-modified poly(ethyleneimine) (PEI-Mal) on primary human mesenchymal stem cell, harvested from reaming debris (rdMSC) and osteoblasts obtained from four different male donors. PEI-Mal-nanoparticles with two different molecular weights of the PEI core (5000 g/mol for PEI-5k-Mal-B and 25,000 g/mol for PEI-25k-Mal-B) have been administered to both cell lines. As well dose as incubation-time dependent effects and interactions have been researched for concentrations between 1 μg/ml to 1 mg/ml and periods of 24 h up to 28 days. Studies conducted by different methods of microscopy as light microscopy, fluorescence microscopy, transmission-electron-microscopy and quantitative assays (LDH and DC-protein) indicate as well a good cellular uptake of the nanoparticles as a particle- and concentration-dependent impact on the cellular macro- and micro-structure of the rdMSC samples. In all experiments PEI-5k-Mal-B exhibits a superior biocompatibility compared to PEI-25k-Mal-B. At higher concentrations PEI-25k-Mal-B is toxic and induces a directly observable mitochondrial damage. The alkaline phosphatase assay (ALP), has been conducted to check on the possible influence of nanoparticles on the differentiation capabilities of rdMSC to osteoblasts. In addition the production of mineralized matrix has been shown by von-Kossa stained samples. No influence of the nanoparticles on the ALP per cell has been detected. Additionally, for all experiments, results are strongly influenced by a large donor-to-donor variability of the four different rdMSC samples. To summarize, while featuring a good cellular uptake, PEI-5k-Mal-B induces only minimal adverse effects and features clearly superior biocompatibility compared to the larger PEI-25k-Mal-B
Effects of dendritic core-shell glycoarchitectures on primary mesenchymal stem cells and osteoblasts obtained from different human donors
The biological impact of novel nano-scaled drug delivery vehicles in highly topical therapies of bone diseases have to be investigated in vitro before starting in vivo trials. Highly desired features for these materials are a good cellular uptake, large transport capacity for drugs and a good bio-compatibility. Essentially the latter has to be addressed as first point on the agenda. We present a study on the biological interaction of maltose-modified poly(ethyleneimine) (PEI-Mal) on primary human mesenchymal stem cell, harvested from reaming debris (rdMSC) and osteoblasts obtained from four different male donors. PEI-Mal-nanoparticles with two different molecular weights of the PEI core (5000 g/mol for PEI-5k-Mal-B and 25,000 g/mol for PEI-25k-Mal-B) have been administered to both cell lines. As well dose as incubation-time dependent effects and interactions have been researched for concentrations between 1 μg/ml to 1 mg/ml and periods of 24 h up to 28 days. Studies conducted by different methods of microscopy as light microscopy, fluorescence microscopy, transmission-electron-microscopy and quantitative assays (LDH and DC-protein) indicate as well a good cellular uptake of the nanoparticles as a particle- and concentration-dependent impact on the cellular macro- and micro-structure of the rdMSC samples. In all experiments PEI-5k-Mal-B exhibits a superior biocompatibility compared to PEI-25k-Mal-B. At higher concentrations PEI-25k-Mal-B is toxic and induces a directly observable mitochondrial damage. The alkaline phosphatase assay (ALP), has been conducted to check on the possible influence of nanoparticles on the differentiation capabilities of rdMSC to osteoblasts. In addition the production of mineralized matrix has been shown by von-Kossa stained samples. No influence of the nanoparticles on the ALP per cell has been detected. Additionally, for all experiments, results are strongly influenced by a large donor-to-donor variability of the four different rdMSC samples. To summarize, while featuring a good cellular uptake, PEI-5k-Mal-B induces only minimal adverse effects and features clearly superior biocompatibility compared to the larger PEI-25k-Mal-B
Transport by molecular motors in the presence of static defects
The transport by molecular motors along cytoskeletal filaments is studied
theoretically in the presence of static defects. The movements of single motors
are described as biased random walks along the filament as well as binding to
and unbinding from the filament. Three basic types of defects are
distinguished, which differ from normal filament sites only in one of the
motors' transition probabilities. Both stepping defects with a reduced
probability for forward steps and unbinding defects with an increased
probability for motor unbinding strongly reduce the velocities and the run
lengths of the motors with increasing defect density. For transport by single
motors, binding defects with a reduced probability for motor binding have a
relatively small effect on the transport properties. For cargo transport by
motors teams, binding defects also change the effective unbinding rate of the
cargo particles and are expected to have a stronger effect.Comment: 20 pages, latex, 7 figures, 1 tabl
Long-lived states in synchronized traffic flow. Empirical prompt and dynamical trap model
The present paper proposes a novel interpretation of the widely scattered
states (called synchronized traffic) stimulated by Kerner's hypotheses about
the existence of a multitude of metastable states in the fundamental diagram.
Using single vehicle data collected at the German highway A1, temporal velocity
patterns have been analyzed to show a collection of certain fragments with
approximately constant velocities and sharp jumps between them. The particular
velocity values in these fragments vary in a wide range. In contrast, the flow
rate is more or less constant because its fluctuations are mainly due to the
discreteness of traffic flow.
Subsequently, we develop a model for synchronized traffic that can explain
these characteristics. Following previous work (I.A.Lubashevsky, R.Mahnke,
Phys. Rev. E v. 62, p. 6082, 2000) the vehicle flow is specified by car
density, mean velocity, and additional order parameters and that are
due to the many-particle effects of the vehicle interaction. The parameter
describes the multilane correlations in the vehicle motion. Together with the
car density it determines directly the mean velocity. The parameter , in
contrast, controls the evolution of only. The model assumes that
fluctuates randomly around the value corresponding to the car configuration
optimal for lane changing. When it deviates from this value the lane change is
depressed for all cars forming a local cluster. Since exactly the overtaking
manoeuvres of these cars cause the order parameter to vary, the evolution
of the car arrangement becomes frozen for a certain time. In other words, the
evolution equations form certain dynamical traps responsible for the long-time
correlations in the synchronized mode.Comment: 16 pages, 10 figures, RevTeX
Adhesion of membranes via receptor-ligand complexes: Domain formation, binding cooperativity, and active processes
Cell membranes interact via anchored receptor and ligand molecules. Central
questions on cell adhesion concern the binding affinity of these
membrane-anchored molecules, the mechanisms leading to the receptor-ligand
domains observed during adhesion, and the role of cytoskeletal and other active
processes. In this review, these questions are addressed from a theoretical
perspective. We focus on models in which the membranes are described as elastic
sheets, and the receptors and ligands as anchored molecules. In these models,
the thermal membrane roughness on the nanometer scale leads to a cooperative
binding of anchored receptor and ligand molecules, since the receptor-ligand
binding smoothens out the membranes and facilitates the formation of additional
bonds. Patterns of receptor domains observed in Monte Carlo simulations point
towards a joint role of spontaneous and active processes in cell adhesion. The
interactions mediated by the receptors and ligand molecules can be
characterized by effective membrane adhesion potentials that depend on the
concentrations and binding energies of the molecules.Comment: Review article, 13 pages, 9 figures, to appear in Soft Matte
Transport of Beads by Several Kinesin Motors
The movements of beads pulled by several kinesin-1 (conventional kinesin) motors are studied both theoretically and experimentally. While the velocity is approximately independent of the number of motors pulling the beads, the walking distance or run-length is strongly increased when more motors are involved. Run-length distributions are measured for a wide range of motor concentrations and matched to theoretically calculated distributions using only two global fit parameters. In this way, the maximal number of motors pulling the beads is estimated to vary between two and seven motors for total kinesin concentrations between 0.1 and 2.5 μg/ml or between 0.27 and 6.7 nM. In the same concentration regime, the average number of pulling motors is found to lie between 1.1 and 3.2 motors
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